Population Genetics Analysis

Insthoviricetes

Phylogenetic Tree

DNA Polymorphism

Genera	Eta	h	Hd	Pi	Theta (per site) from Eta	k
Alphainfluenzavirus	7802	24	1	0.16355	0.16347	2090.395
Betainfluenzavirus	1964	20	1	0.04168	0.04029	572.642
Gammainfluenzavirus	996	4	0.9	0.03479	0.03877	428.9
Deltainfluenzavirus	1242	18	0.989	0.02307	0.02865	281.811
Influenza virus	13964	66	0.999	0.39641	0.29528	3902.273
Orthomyxoviridae	4584	82	0.999	0.42631	0.46679	833.004
Articulavirales	3722	83	0.999	0.42255	0.47273	660.439
Insthoviricetes	3722	83	0.999	0.42255	0.47273	660.439

1. Total Number of Mutations, Eta: Refers to the total count of mutations observed across a set of DNA sequences, providing a measure of genetic variability within a population.

2. Number of Haplotypes, h: The count of distinct haplotypes (a collection of specific genes or DNA sequences in an individual) detected in a sample, indicating genetic structure and diversity.

3. Haplotype Diversity, Hd: A measure of the variation of haplotype frequencies within a population, with values closer to 1 indicating higher diversity.

4. Nucleotide Diversity, Pi: An estimate of the average number of nucleotide differences per site between two sequences, higher values suggest greater genetic diversity.

5. Theta (per site) from Eta: An estimate based on the total number of mutations, used to measure the level of genetic variation per nucleotide site in a population.

6. Average Number of Nucleotide Differences, k: The average number of nucleotide differences observed between pairs of sequences, reflecting the average genetic distance within a population.

Gene Flow

Genera	S	h	Hd	K	Pi	PiJC
Alphainfluenzavirus	554	23	0.9964	206.5906	0.1322	0.1476
Betainfluenzavirus	192	20	1.0000	63.5790	0.0407	0.0425
Gammainfluenzavirus	98	4	0.9000	41.6000	0.0266	0.0273
Deltainfluenzavirus	145	18	0.9895	33.5737	0.0215	0.0219
Others	1513	18	1.0000	824.8105	0.5277	1.0004

1. Number of Segregating Sites, S: The total count of variable positions in a set of DNA sequences where at least one sequence differs from the others. It is an indicator of genetic variation within a population.

2. Number of Haplotypes, h: The count of unique haplotypes identified within a sample, which are specific combinations of DNA sequences. It is an indicator of genetic diversity within a population.

3. Haplotype Diversity, Hd: A measure of the diversity in the distribution of haplotype frequencies within a population, varying from 0 to 1, with values closer to 1 indicating higher haplotype diversity.

4. Average Number of Differences, K: The average number of nucleotide differences observed between every pair of sequences in a set, which can be used to estimate genetic distance within a population.

5. Nucleotide Diversity, Pi: A measure of the average base diversity or differences at each position in a set of DNA sequences. Higher Pi values indicate greater genetic diversity.

6. Nucleotide Diversity with JC, PiJC: Nucleotide diversity adjusted using the Jukes-Cantor (JC) model, which accounts for potentially unrecognized multiple substitutions at the same site, providing a more accurate estimate of genetic diversity.

Genetic Differentiation

Genera	Alphainfluenzavirus	Betainfluenzavirus	Gammainfluenzavirus	Deltainfluenzavirus	Others
Alphainfluenzavirus		686.06875	763.36667	771.14792	849.01157
Betainfluenzavirus	686.06875		685.75	686.4825	874.95833
Gammainfluenzavirus	763.36667	685.75		495.68	888.71111
Deltainfluenzavirus	771.14792	686.4825	495.68		897.40278
Others	849.01157	874.95833	888.71111	897.40278

"Others" refers to all other genera within Insthoviricetes, excluding Alphainfluenzavirus, Betainfluenzavirus, Gammainfluenzavirus, and Deltainfluenzavirus.